Tissue Dissociation References


	Tissue Dissociation References
	Tissue Type: Uterus

Uterus: Mouse
BALB/c mice
	Bigsby, R., Cooke, P., and Cunha, G.: A Simple Efficient Method For Separating Murine Uterine Epithelial and Mesenchymal Cells, Am J Physiol 251, E630, 1986
Cells:	Epithelial cellsMesencymal cells
Enzyme(s)	Trypsin: 1% Soybean Trypsin Inhibitor: 1% Deoxyribonuclease: 0.1%
Medium	DMEM
Uterus: Mouse
40 day old BALB/cCrgl mice
	Cooke, P., Uchima, F., Fujii, D., Bern, H., and Cunha, G.: Restoration of Normal Morphology and Estrogen Responsiveness in Cultured Vaginal and Uterine Epithelia Transplanted with Stroma, Proc Natl Acad Sci U S A 83, 2109, 1986
Cells:	Epithelial cells
Enzyme(s)	Collagenase Type III: 0.1% Trypsin: 5 mg
Medium	Medium 199
Uterus: Mouse
21-23 day old outbread CD-1 mice
	Tomooka, Y., DiAugustine, R., and McLachlan, J.: Proliferation of Mouse Uterine Epithelial Cells in Vitro, Endocrinology 118 (3), 1011, 1986
Cells:	Epithelial cells
Enzyme(s)	Trypsin
Medium	Medium 199
Uterus: Rat
Female, SD rats
	Glasser, S., Julian, J., Decker, G., Tang, J., and Carson, D.: Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells, J Cell Biol 107 (6), 2409, 1988
Cells:	Luminal epithelial cells
Enzyme(s)	Trypsin
Medium	HBSS